iuvo Microconduit Arrays enable the use of highly miniaturized, advanced cell models and functional assays with automated
liquid handling and HCA platforms, with the goal of more accurate replication of in vivo processes in drug discovery. Instead
of the "buckets" of various sizes used for cell culture in multiwell plates, iuvo plates have cell culture compartments with
geometries designed specifically to support the biology and/or functions of interest.
uvo plates comply with SBS standards, and the liquid in the microchannels can be displaced with passive pumping as often as
necessary using standard automated liquid dispensing equipment. No specialized instrumentation is required to carry out
assays or acquire the data. Three dimensional cell culture becomes easy to carry out, and the entire compartment is
accessible to microscope-based HCA instruments as well as plate scanners. The microchannel environment reduces dilution of
secreted signaling molecules, and the media can be sampled for analysis of secreted factors.
Surface tension leads to pressure inside drops. All things equal, the pressure is higher inside smaller drops (~1/r). When
two drops are connected by a channel, the pressure is equilibriated by flow. The smaller drop flows into the channel,
replacing the channel contents.
The importance of 3D extracellular matrix on cellular function and behavior is clear from studies in differentiation, tissue
engineering and even molecular pharmacology. BellBrook Labs is leveraging its unique iuvo™ Microconduit Array technology to
provide high content cellular assays in 3D extracellular matrix. Let BellBrook Labs Bring Its 3D Expertise & Experience to
your Research!
* Screening/Profiling in 3D Biological Matrix - Replicates in vivo microenvironment and signaling pathways.
* Quantitative Compound Data - Quantitative potency data including IC50 and magnitude of effect. In situ molecular
staining allows pathway and mechanism analysis. Final reports include raw image files, dose response curves, and IC50 values.
* Multiparametric High Content Platform - Allows a variety of assays, including viability, cell cycle, apoptosis, and
migration/invasion.
* Rapid Turn-Around Time - Data delivered within three weeks.
Invasion of tumor cells through ECM (extracellular matrix) is one of the hallmarks of cancer, yet the process is poorly
modeled in vitro, especially in an automated format. We are now offering compound profiling services for information rich, 3D
tumor cell invasion assays using our novel iuvo Microchannel 5250 array platform. Designed as an improvement to Boyden
chamber assays, our fully automated invasion assay uses microscopic imaging of cell movement through horizontally-oriented,
collagen filled microchannels to provide quantitative data on cell motility. The microchannels facilitate post-assay
staining, which dramatically increases the information content of the assays.
liquid handling and HCA platforms, with the goal of more accurate replication of in vivo processes in drug discovery. Instead
of the "buckets" of various sizes used for cell culture in multiwell plates, iuvo plates have cell culture compartments with
geometries designed specifically to support the biology and/or functions of interest.
uvo plates comply with SBS standards, and the liquid in the microchannels can be displaced with passive pumping as often as
necessary using standard automated liquid dispensing equipment. No specialized instrumentation is required to carry out
assays or acquire the data. Three dimensional cell culture becomes easy to carry out, and the entire compartment is
accessible to microscope-based HCA instruments as well as plate scanners. The microchannel environment reduces dilution of
secreted signaling molecules, and the media can be sampled for analysis of secreted factors.
Surface tension leads to pressure inside drops. All things equal, the pressure is higher inside smaller drops (~1/r). When
two drops are connected by a channel, the pressure is equilibriated by flow. The smaller drop flows into the channel,
replacing the channel contents.
The importance of 3D extracellular matrix on cellular function and behavior is clear from studies in differentiation, tissue
engineering and even molecular pharmacology. BellBrook Labs is leveraging its unique iuvo™ Microconduit Array technology to
provide high content cellular assays in 3D extracellular matrix. Let BellBrook Labs Bring Its 3D Expertise & Experience to
your Research!
* Screening/Profiling in 3D Biological Matrix - Replicates in vivo microenvironment and signaling pathways.
* Quantitative Compound Data - Quantitative potency data including IC50 and magnitude of effect. In situ molecular
staining allows pathway and mechanism analysis. Final reports include raw image files, dose response curves, and IC50 values.
* Multiparametric High Content Platform - Allows a variety of assays, including viability, cell cycle, apoptosis, and
migration/invasion.
* Rapid Turn-Around Time - Data delivered within three weeks.
Invasion of tumor cells through ECM (extracellular matrix) is one of the hallmarks of cancer, yet the process is poorly
modeled in vitro, especially in an automated format. We are now offering compound profiling services for information rich, 3D
tumor cell invasion assays using our novel iuvo Microchannel 5250 array platform. Designed as an improvement to Boyden
chamber assays, our fully automated invasion assay uses microscopic imaging of cell movement through horizontally-oriented,
collagen filled microchannels to provide quantitative data on cell motility. The microchannels facilitate post-assay
staining, which dramatically increases the information content of the assays.
No comments:
Post a Comment